New Zealand Plant Protection 69 (2016): 328
Apple exports intercepted with cocooning apple leafcurling midge (ALCM) are fumigated with methyl bromide (MB), which reduces apple quality. ALCM is controlled in the orchard using the insecticide Movento™, while also being parasitised by Platygaster demades and preyed on by generalist predators such as Sejanus albisignata. These ALCM control measures make it difficult to collect sufficient numbers of ALCM-infested apples for disinfestation research on alternatives to MB. Therefore, procedures were developed for collecting and storing cocooning ALCM on apples in the laboratory. ALCM-infested leaves were collected in plastic bags, saturated with water and placed at 20°C. The water stimulated larvae to exit the leaf-rolls. Using a fine paint brush, ten larvae were placed on the calyx of an apple and confined using a cage attached with Blu-Tak™. The apples were placed in plastic bins and sprayed with water. A mesh net and a brown paper bag were secured over the top of the bin to reduce condensation. The bin was stored at 20°C, 16:8 light:dark for 10-15 days. Of 1,133 apples established, 988 of them (87.2 %) had on average 2.2 cocooning ACLM. Large numbers of cocooning ALCM on fruit can now be provided for disinfestation research.
Copyright © 2016 New Zealand Plant Protection Society (Inc.).