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New Zealand Plant Protection 68 (2015): 332-339

In vitro inhibition of Pseudomonas syringae pv. actinidiae by wound protectants

D.A. Cornish, J. Yu, J.M. Oldham, J. Benge, W. Max and J.L. Vanneste

ABSTRACT

Preventing Pseudomonas syringae pv. actinidiae (Psa), the causal agent of bacterial canker of kiwifruit, from entering pruning wounds would reduce disease incidence and facilitate canopy management. Antibacterial activity against Psa has been determined in the laboratory for active ingredients commercially available in New Zealand. Only tebuconazole, tebuconazole with octhilinone and a formulation of cyproconazole and iodocarb killed Psa on agar plates or in liquid medium. However, only tebuconazole with octhilinone killed Psa within 5 min. Psa was detected by polymerase chain reaction following plating on instruments used for applying wound protectants in an infected orchard. This suggests that under some conditions the pathogen could be transferred from vine to vine when applying some wound protectants. Addition of streptomycin sulphate to tebuconazole or a formulation of cyproconazole and iodocarb did not result in a rapid and complete kill of Psa similar to that with a commercial formulation of streptomycin applied alone.

Keywords: bacterial canker of kiwifruit, pruning paste, streptomycin.

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