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New Zealand Plant Protection 67 (2014): 220-225

Using qPCR to monitor populations of Pseudomonas syringae pv. actinidiae on kiwifruit vines

I.P.S. Pushparajah, T.R. Ryan, L.G. Hawes, B.N. Smith, G.B. Follas, J. Rees-George and K.R. Everett

ABSTRACT

Populations of Pseudomonas syringae pv. actinidiae (Psa) on vines sprayed with a biological control agent (Bacstar™; Bacillus amyloliquefaciens D747; Ba) and on untreated control vines were monitored using qPCR. Leaf discs were taken from vines in February 2014, 3 months after the final of three spray applications of Bacstar™ in October and November 2013. An aliquot of leaf washings in bacterial saline (BS) was spread on to potato dextrose agar in Petri plates and the numbers of colonies of Ba were counted. DNA was extracted from the remaining BS and the quantity of Psa DNA was determined using qPCR. Ba was recovered from leaf discs that had been sprayed with Bacstar™ in greater numbers than from unsprayed leaf discs (P<0.05). Psa was recovered in greater numbers from the

Keywords: field trials, Psa, Bacillus amyloliquefaciens strain D747.

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