New Zealand Plant Protection 67 (2014): 213-219

Spraying hydroponic lettuce roots with a suspension concentrate formulation of Bacillus velezensis to suppress root rot disease and promote plant growth

M. Kanjanamaneesathian, R. Wiwattanapatapee, W. Rotniam and W. Wongpetkhiew

ABSTRACT

Bacillus velezensis was prepared as a suspension concentrate (SC) and sprayed on to the roots of 1-month-old Lactuca sativa, grown in a hydroponic system. In the first experiment using Lactuca sativa (var. Red Coral) with root rot symptoms, a 1% blank with no cells, a 1% SC (10¹³ CFU/ml) and 1% fresh bacterial cells (FC) (10¹⁴ CFU/ml) reduced the percentage of root tips colonized by the pathogen and increased the fresh shoot weight compared to plants that were not sprayed. In the second experiment with L. sativa (var. Green Oak) using healthy-looking roots, a 1% blank, 10% SC (10¹³ CFU/ml) and 1% FC of the bacterium (10¹⁴ CFU/ml) reduced the percentage of root tips colonized by Pythium sp. The SC and FC of the bacterium increased the fresh shoot weight, while the blank, the SC and the FC of the bacterium increased the dried weight of the shoot.

Keywords: Bacillus velezensis, hydroponic vegetables, root rot disease, suspension concentrate.

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