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New Zealand Plant Protection 67 (2014): 86-95

Towards a test to verify that wood has been heat-treated to the ISPM15 standard

I.I. Iline, M.A. Novoselov, N.K. Richards and C.B. Phillips

ABSTRACT

International trade of wood, including dunnage, is a well-known pathway for spreading diseases and wood-boring insects between countries. To mitigate this risk, the International Standard for Phytosanitary Measures No. 15 (ISPM15) states that wood to confirm that wood has been properly heat-treated. After preliminary investigations of 12 enzymes and 3 sugars from pine xylem, experiments focused on malate dehydrogenase (MDH) and fructose. Samples from the surfaces of heat-treated Pinus radiata wood exhibited decreased MDH activity and increased fructose concentration. However, samples from 5 mm deeper in the profile of heated wood showed similar MDH activity to unheated wood, but contained relatively lower fructose concentrations. There is potential to exploit heatinduced changes in MDH activity and fructose concentration to develop a quick, easily-used assay for verifying compliance of wood packaging materials with ISPM15.

Keywords: heat treatment, ISPM15, Pinus radiata, xylem metabolites, malate dehydrogenase, fructose, wood packaging material.

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