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New Zealand Plant Protection 66 (2013): 378

Development of citrophilus mealybug (Pseudococcus calceolariae) on grape roots

W.R.M. Sandanayaka, V.A. Davis and D. Cohen

ABSTRACT

Pseudococcus calceolariae (Hemiptera: Pseudococcidae) is a vector of Grapevine leafroll-associated virus 3 (GLRaV-3), the most destructive virus disease affecting New Zealand grapevines. Previous studies suggest that P. calceolariae can survive on remnant vine roots for at least 12 months and these mealybugs could account for the spread of the disease in replanted vineyards. A laboratory assay test was developed to observe the growth of P. calceolariae on grape rootstock 3309. Clean root pieces were left on moist filter paper in Petri dishes for a week to encourage new growth before P. calceolariae eggs (n=15) were inoculated and held at 23C. Recordings were made every 3-4 days of the survival and development from first instar until males emerged as adults and females started oviposition. About 50% mortality was recorded during first and second instars and ca 30% developed into adult stages. The development periods from first instar to adult males and females were 44.1 1 (n=16) and 45.4 1 (n=22) respectively. This methodology is being developed to assess the relative resistance of grapevine rootstocks, effects of contact insecticides and to understand GLRaV-3 transmission from roots.

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