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New Zealand Plant Protection 66 (2013): 365-373

The impact of post acquisition period on detection of Candidatus Liberibacter solanacearum in tomato potato psyllid

W.R.M. Sandanayaka, L.K. Tooman and R.E. Hewett

ABSTRACT

The impact of post acquisition period (PAP) on detection of Candidatus Liberibacter solanacearum (Lso) in adult tomato potato psyllid (TPP; Bactericera cockerelli) was investigated using qPCR assays on whole insects. Lso in adults was initially tested immediately after acquisition access periods (AAP) of 15, 24, 48, 72, 96 h or 1 week on Lso-infected tomato leaves. These results were compared with those from adults with 15 or 24 h AAP followed by 24-, 48- or 72-h PAP on Lso-free tomato leaves. From 15-20 individuals of each treatment tested, 5-15% were positive for Lso immediately after AAP of 15 or 24 h. That increased to 84% and 100% with 24- and 48-h PAPs respectively, but decreased to 25-47% after 72 h. Longer AAP (48 h-1 week) without PAP did not exceed 55% acquisition success. The phloem ingestion and possible acquisition of Lso during 15 and 24 h plant access periods are discussed.

Keywords: tomato/potato psyllid, Candidatus Liberibacter solanacearum, post acquisition period, phloem ingestion, detection.

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