New Zealand Plant Protection 66 (2013): 254-258
Downy mildew of boysenberry is caused by the biotrophic pathogen Peronospora sparsa. To ensure supplies of viable spore inoculum for infection experiments, six storage methods were assessed: the leaf discs cut from sporulating areas of the leaf stored dry or in 20% glycerol, and spores were suspended in 20% glycerol, all three of which were stored at either -20°C or -80°C. After 1, 2, 4 or 6 months storage, spore viability and the capability to infect leaf discs were evaluated. Storage methods had no significant effects on spore germination or infection. Storage time and temperature significantly (P<0.001) affected spore viability and infection, being greatest after 1 month and at -80°C. Overall, viability of spores stored as suspensions at -80°C (the most effective treatment) was greater than all other methods tested, and spore germination decreased from over 60% after 1 month of storage to less than 5% after 4 months.
Keywords: storage temperature, spore germination, infection, sporulating, dryberry.
|Evaluation of methods for sterilising boysenberry leaves for downy mildew infection studies|
A.M. Herath Mudiyanselage, M.V. Jaspers, H.J. Ridgway, M. Walter, G.I. Langford and E.E. Jones (2012)
New Zealand Plant Protection 65: 297
|The use of a PCR diagnostic test to predict and control Peronospora sparsa, downy mildew of boysenberry|
S.L. Dodd, K.S.H. Boyd-Wilson, D. Shanmuganathan and M. Walter (2007)
New Zealand Plant Protection 60: 306
Copyright © 2013 New Zealand Plant Protection Society (Inc.).