Journal home   ·   This volume   ·   Previous abstract   ·   Next abstract

New Zealand Plant Protection 66 (2013): 254-258

Evaluation of methods for long term storage of the boysenberry downy mildew pathogen Peronospora sparsa

A.M. Herath Mudiyanselage, M.V. Jaspers, H.J. Ridgway, M. Walter, G.I. Langford and E.E. Jones

ABSTRACT

Downy mildew of boysenberry is caused by the biotrophic pathogen Peronospora sparsa. To ensure supplies of viable spore inoculum for infection experiments, six storage methods were assessed: the leaf discs cut from sporulating areas of the leaf stored dry or in 20% glycerol, and spores were suspended in 20% glycerol, all three of which were stored at either -20C or -80C. After 1, 2, 4 or 6 months storage, spore viability and the capability to infect leaf discs were evaluated. Storage methods had no significant effects on spore germination or infection. Storage time and temperature significantly (P<0.001) affected spore viability and infection, being greatest after 1 month and at -80C. Overall, viability of spores stored as suspensions at -80C (the most effective treatment) was greater than all other methods tested, and spore germination decreased from over 60% after 1 month of storage to less than 5% after 4 months.

Keywords: storage temperature, spore germination, infection, sporulating, dryberry.

Related articles
pdfEvaluation of methods for sterilising boysenberry leaves for downy mildew infection studies
A.M. Herath Mudiyanselage, M.V. Jaspers, H.J. Ridgway, M. Walter, G.I. Langford and E.E. Jones (2012)
New Zealand Plant Protection 65: 297
pdfThe use of a PCR diagnostic test to predict and control Peronospora sparsa, downy mildew of boysenberry
S.L. Dodd, K.S.H. Boyd-Wilson, D. Shanmuganathan and M. Walter (2007)
New Zealand Plant Protection 60: 306

Copyright © 2013 New Zealand Plant Protection Society (Inc.).

Please refer to the terms of use.