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New Zealand Plant Protection 64 (2011): 292

Screening of historical isolates stored in New Zealand culture collections for Pseudomonas syringae pv. actinidiae

D. Park, D. Than, K.R. Everett, J. Rees-George, M.K. Romberg and B.J.R. Alexander

ABSTRACT

Subsequent to the detection of Pseudomonas syringae pv. actinidiae (Psa) on kiwifruit in Te Puke (November 2010), isolates of Pseudomonas spp. from kiwifruit stored in the International Collection of Microorganisms from Plants (ICMP) and at Plant & Food Research (PFR) were tested by PCR for Psa. A total of 139 isolates from ICMP were assayed with the previously published primer pairs PsaF1/PsaR2 and PAV1/P22. An amplicon of expected size for Psa was produced from 19 isolates using primers PAV1/P22, but no amplicons were obtained using the primers PsaF1/PsaR2. Amplicons produced by primers PAV1/P22 were sequenced and these isolates were identified as Pseudomonas viridiflava. A further 52 Pseudomonas isolates collected from kiwifruit and stored at PFR did not amplify a PCR product with primers PsaF1/PsaR2. These PCR tests confirm that Psa was not detected in any of the cultures held in the ICMP or at PFR. The screening of historical Pseudomonas isolates associated with kiwifruit suggests that Psa strains had not been isolated from New Zealand kiwifruit samples prior to the detection in 2010.

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