New Zealand Plant Protection 64 (2011): 291
The thermal death point was determined in vitro for three isolates of Pseudomonas syringae pv. actinidiae (Psa). A bacterial suspension was treated by placing in an Eppendorf tube then floating in a water bath at seven temperatures, from 26.3 to 60°C. At each temperature an aliquot was removed after 5, 15, 35, 75, 155, 315 and 635 min, placed onto King's medium B (KMB) in Petri plates and spread with a sterilised bent glass rod. Petri plates were placed at 25°C for 24 h and colonies counted. There were three isolate replicates each in an Eppendorf tube per time:temperature combination. There was no difference in response between isolate replicates, but time and temperature differences were significant (P<0.0001). Any time:temperature combination greater than 45°C for 5 min killed Psa. Modelling the thermal response suggested that 42.5°C for longer than 150 min was the lowest time:temperature combination that could be used to kill the bacterial cells.
|Heat treatments to kill Pseudomonas syringae pv. actinidiae on contaminated pollen|
K.R. Everett, D. Cohen, I.P.S. Pushparajah, M.J. Vergara, C.L. Curtis, N.J. Larsen and Y. Jia (2012)
New Zealand Plant Protection 65: 8-18
Copyright © 2011 New Zealand Plant Protection Society (Inc.).